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KMID : 0903519910340040403
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1991 Volume.34 No. 4 p.403 ~ p.403
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Studies on the expression mechanism of light - induced gene of rice
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Choi Yang-Do
Kang Yun-Jung
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Abstract
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To study the expression regulatory mechanism of Light-induced gene of rice, cDNA clone for the light harvesting chlorophyll a/b binding protein (Cab) have been isolated. A cDNA library was constructed with mRNA isolated from the leaves of rice seedling grown in the light. A cDNA clone for light response gene was isolated by differiential screening with radio-labeled cDNA probe. Southern blot analysis demonstrated that the clone, λAlA, was 472 bp long. The clone encodes the 3´ region of the Cab. Nucleotide sequencing of the clone reveals that the clone encodes 320 by of coding region and 152 nucleotides of 3´ non-coding sequences.
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